TRIM38 Induced in Respiratory Syncytial Virus-infected Cells Downregulates Type I Interferon Expression by Competing with TRIM25 to Bind RIG-I.

Innate immune response is the first line of defense for the host against virus invasion. One important response is the synthesis and secretion of type I interferon (IFN-I) in the virus-infected host cells. Here, we found that respiratory syncytial virus (RSV) infection induced high expression of TRIM25, which belongs to the tripartite motif-containing (TRIM) family of proteins. TRIM25 bound and activated retinoic acid-inducible gene I (RIG-I) by K63-linked ubiquitination. Accordingly, RIG-I mediated the production of IFN-I mainly through the nuclear factor kappa-B (NF-κB) pathway in respiratory epithelial cells. Interestingly, IFN-I, in turn, promoted a high expression of TRIM38 which downregulated the expression of IFN-I by reducing the protein level of RIG-I by K48-linked ubiquitination. More importantly, the binding site of TRIM25 to RIG-I was found in the narrow 25th-43rd amino acid (aa) region of RIG-I N-terminus. In contrast, the binding sites of TRIM38 to RIG-I were found in a much wider amino acid region, which included the binding site of TRIM25 on RIG-I. As a result, TRIM38 inhibits the production of IFN-I by competing with TRIM25 for RIG-I binding. Thus, TRIM38 negatively regulates RIG-I activation to, in turn, downregulate IFN-I expression, thus interfering with host immune response. A negative feedback loop effectively "puts the brakes" on the reaction once host immune response is overactivated and homeostasis is unbalanced. We also discovered that TRIM25 bound RIG-I by a new K63-linked ubiquitination located at K-45 of the first caspase recruitment domain (CARD). Collectively, these results confirm an antagonism between TRIM38 and TRIM25 in regulating IFN-I production by affecting RIG-I activity following RNA virus infection.

The nonstructural protein 1 of respiratory syncytial virus hijacks host mitophagy as a novel mitophagy receptor to evade the type I IFN response in HEp-2 cells.

Mitochondria are good targets for viruses to manipulate their hosts. However, it remains obscure whether respiratory syncytial virus (RSV) target mitochondria to suppress the type I interferon (IFN) responses. Here, we show that nonstructural protein 1 (NS1) protein of RSV interacts with Tu translation elongation factor mitochondrial (TUFM), which can lead to its localization in mitochondria and finally induce TUFM-dependent mitophagy and inhibition of IFNß. Mechanically, NS1-mediated TUFM-dependent mitophagy does not depend on the PINK1-PARKIN pathway and classic mitophagy receptors. Importantly, NS1 may act as a new receptor protein to bridge mitochondria and autophagosomes by interacting with TUFM and LC3B. The LIR motif of NS1 protein is essential for its interaction with LC3B and is of great importance for its mitophagy induction and IFNß suppression. Finally, NS1-induced TUFM-dependent mitophagy was essential for its attenuated IFNß response using autophagy-deficient cells and mice. Our study provides a novel mitophagy receptor molecular and a new antiviral option by suppressing antiviral innate immune via targeting TUFM-dependent mitophagy. IMPORTANCE It is a worthy concern for us to understand virus-host interactions which affect progression and prognosis of disease. We demonstrated that the non-structural protein 1 of respiratory syncytial virus (RSV NS1) may act as a novel mitophagy receptor to induce mitophagy by binding LC3B and mitochondrial protein TUFM, and finally dampen interferon (IFN) responses induced by RIG1 and RSV infection. TUFM is beneficial for RSV replication in vivo and vitro. It is new and interesting that RSV NS1 may function as a mitophagy receptor to interact with LC3B. The LIR motif of NS1 protein is essential for its interaction with LC3B. We further confirm that RSV NS1 inhibited IFNß response and promoted RSV replication in autophagy-dependent mechanisms in vivo and vitro. Our study contributes to understanding virus-host interaction, enriching our insights into RSV pathogenic mechanism and exploiting new antiviral treatments targeting TUFM.

Ultrathin All-Angle Hyperbolic Metasurface Retroreflectors Based on Directed Routing of Canalized Plasmonics.

Retroreflectors that can accurately redirect the incident waves in free space back along their original channels provide unprecedented opportunities for light manipulation. However, to the best of our knowledge, they suffer from either the bulky size, narrow angular bandwidths, or time-consuming postprocessing, which essentially limits their further applications. Here, a scheme for designing ultrathin all-angle real-time retroreflectors based on hyperbolic plasmonic metasurfaces is proposed and experimentally demonstrated. The physical mechanism underlying the scheme is the orthogonality between the traveling waves in free space and the canalized spoof surface plasmon on the hyperbolic plasmonic metasurfaces, which guarantee their high-efficiency and all-angle mutual conversion. In this case, the strong confinement characteristic that benefited from the enhanced light-matter interaction enables us to route and retroreflect the canalized spoof surface plasmon with extremely thin structures. As proof of the scheme, a retroreflector prototype with a thickness approximately equal to the central wavelength is designed and fabricated. Further experimental investigation obtains a half-power field of view up to 53° and a maximum efficiency of 83.2%. This scheme can find promising applications in target detection, remote sensing, and diverse on-chip light control devices.

Terahertz non-label subwavelength imaging with composite photonics-plasmonics structured illumination.

Inspired by the capability of structured illumination microscopy (SIM) in subwavelength imaging, many researchers devoted themselves to investigating this methodology. However, due to the free-propagating feature of the traditional structured illumination fields, the resolution can be only improved up to two-fold of the diffraction-limited microscopy. Besides, most of the previous studies, relying on incoherent illumination sources, are restricted to fluorescent samples. In this work, a subwavelength non-fluorescent imaging method is proposed based on the illumination of terahertz traveling waves and plasmonics. Excited along with a metal grating, the spoof surface plasmons (SSPs) are employed as one of the illuminating sources. When the scattering waves with the SSPs illumination are captured, the sample's high-order spatial frequencies (SF) components are already encoded into the obtainable low-order ones. Then, a modified post-processing algorithm is exploited to shift the modulated SF components to their actual positions in the SF domain. In this manner, the fine information of samples is introduced to reconstruct the desired imaging, leading to an enhancement of the resolution up to 0.12λ0. Encouragingly, the resolution can be further enhanced by attaching extra illumination of SSPs with an elaborately selected frequency. This method holds promise for some important applications in terahertz non-fluorescent microscopy and sample detection with weak scattering.

Terahertz subwavelength edge detection based on dispersion-induced plasmons.

Terahertz imaging has recently attracted great attention owing to the abilities of high penetration and low ionizing damages. However, the low resolution and low contrast resulting from the diffraction limit and unwanted background illumination significantly hinder the extensive usage. In this Letter, we propose and numerically demonstrate a terahertz subwavelength imaging method capable of extracting only the edges and fine features of the targets. The underlying physics is the efficient transmission of the scattering evanescent waves related to key geometric information while blocking the propagating components. By exploiting the structurally induced plasmons in a bounded metallic waveguide, the transmission channel for evanescent waves is realized by hyperbolic metamaterials through periodically stacking dielectric layers. On this basis, high-contrast edge detection with a resolution up to ${0.1}\lambda$ is demonstrated at terahertz wavelengths. The proposed terahertz imaging method may find important applications in non-destructive testing, weak scattering object detection, and high-contrast microscopy.

Bifunctional Luneburg-fish-eye lens based on the manipulation of spoof surface plasmons.

Manipulation of spoof surface plasmons (SSPs) has recently intrigued enormous interest due to the capability of guiding waves with subwavelength footsteps. However, most of the previous studies, manifested for a single functionality, are not suitable for multifunctional integrated devices. Herein, a bifunctional Luneburg-fish-eye lens is proposed based on a 2D metal pillar array. First, by tuning the dimension of the metal pillars in the array, its ability to precisely manipulate the SSPs along one direction is confirmed, achieving subwavelength focusing and imaging with a resolution up to 0.14λ. Then, separately controlling the propagation of the SSPs along the orthotropic directions is further implemented, and the bifunctional Luneburg-fish-eye lens is realized. It is experimentally characterized as a Luneburg lens along the x axis, whereas in the y axis, it presents the properties of a Maxwell fish-eye lens. This bifunctional lens can reduce the system complexity and exert flexibility in multifunctional applications, while the proposed metal pillar-based design method broadens the application range of the gradient refractive-index lens in microwave, terahertz, and even optical ranges.

High-efficiency directional excitation of spoof surface plasmons by periodic scattering cylinders.

In this Letter, we propose and experimentally demonstrate a simple but efficient method to excite spoof surface plasmons (SSP) through periodic metallic cylinders at microwave frequencies. The rigorous multiple scattering theory indicates that most of the incident propagating waves can pass the cylinders and be converted into the desired harmonics. Furthermore, by tuning the incident angle, controlling the directions of the excited SSP at different frequencies is also realized. The numerical simulations achieve a bidirectional efficiency of 90% at 9.68 GHz and unidirectional efficiency of 79%-85% at 7.46-9.7 GHz, when the incident angle changes from 60° to 120°. Meanwhile, the maximum contrast ratio between the powers of SSP launched in two opposite directions can reach up to 34 dB. The experimental results under 90° and 77.5° illuminations at 9.68 and 8.56 GHz provide strong support for the coupling mechanism. This method may provide technique support in the SSP-based communication and imaging systems.

High-efficiency terahertz spin-decoupled meta-coupler for spoof surface plasmon excitation and beam steering.

Spoof surface plasmon (SSP) meta-couplers that efficiently integrate other diversified functionalities into a single ultrathin device are highly desirable in the modern microwave and terahertz fields. However, the diversified functionalities, to the best of our knowledge, have not been applied to circular polarization meta-couplers because of the spin coupling between the orthogonal incident waves. In this paper, we propose and demonstrate a terahertz spin-decoupled bifunctional meta-coupler for SSP excitation and beam steering. The designed meta-coupler is composed of a coupling metasurface and a propagating metasurface. The former aims at realizing anomalous reflection or converting the incident waves into SSP under the illumination of the right or left circular polarization waves, respectively, and the latter are used to guide out the excited SSP. The respective converting efficiency can reach 82% and 70% at 0.3THz for the right and left circular polarization incident waves. Besides, by appropriately adjusting the reflection phase distribution, many other functionalities can also be integrated into the meta-coupler. Our study may open up new routes for polarization-related SSP couplers, detectors, and other practical terahertz devices.

Terahertz multichannel metasurfaces with sparse unit cells.

Reflective multichannel metasurfaces are flat reflectors that can control incident and reflected waves in a number of propagating directions simultaneously. However, they are always densely discretized with a high spatial resolution, which increases the manufacturing complexity. In this Letter, to the best of our knowledge, a new method that combines the array antenna theory with the metagratings theory is proposed. We demonstrate that the unit cells with a linear gradient phase in each period of the metasurfaces can eliminate specific space harmonics. With this method, multichannel metasurfaces can be designed with sparse unit cells, and high efficiency is maintained simultaneously. As proofs of the method, we design three different terahertz multichannel metasurfaces with no more than three unit cells per period. The simplification of structures can efficiently reduce the manufacturing complexity. This work may open up new routes in designing multichannel metasurfaces.

Superfocusing of terahertz wave through spoof surface plasmons.

In this paper, we propose and numerically demonstrate a new way to realize superfocusing of terahertz waves via the spoof surface plasmons (SSP). With the assist of a modified subwavelength metallic grating, a near-field rapid oscillation can be formed, originating from the Fabry-Perot resonances due to the reflection of SSP waves at terminations. We show that the field pattern of oscillation on textured metallic surface can be engineered by adjusting groove width and grating number. This produces a desired modulation of phase and amplitude for the radiationless electromagnetic interference (REI) focusing. The effective focusing depth through the corrugated metal is evaluated by the full-width-half-maximum (FWHM) beamwidth. At the situation of third-order Fabry-Perot resonance, the FWMH reaches up to 0.069λ at a distance of 0.1λ, improving the beamwidth by more than 540% compared with a single slit. The FWHM is optimized to 0.06λ as the order of Fabry-Perot resonance becomes seven, leading to the superfocusing metric of 1.67. On the basis of this, we further show the focusing ability can be held on the ultra-thin metallic grating. Two-dimensional subwavelength focusing behavior is also numerically verified. Our study may extend the working distance of sensing and super-resolution imaging devices at terahertz frequency.

Experimental demonstration of an ultra-broadband subwavelength resolution probe from microwave to terahertz regime.

An ultra-broadband subwavelength resolution probe that consists of a Teflon rod and six metallic strips is developed for the near-field imaging system. The slit between two metallic strips maintains quasi-TEM modes, avoiding the problem of low coupling efficiency caused by the cutoff effect. The numerical calculations visualize the process of energy compression into a 0.047λ diameter spot with great field enhancement at the taper apex, and the probe holds subwavelength focusing behavior from 10 GHz to 0.25 THz. Although limited by the fabrication, the resolution of 0.16 and 0.25λ are still experimentally demonstrated at 14 GHz and 0.1 THz. The properties of easy fabrication and no cutoff frequency would lower the threshold of a high-resolution near-field imaging system.